Molecular identification of Oncomelania hupensis lindoensis, snail intermediate hosts of Schistosoma japonicum from Central Sulawesi, Indonesia

Abstract. Sutrisnawati, Ramadhan A, Trianto M. 2022. Molecular identification of Oncomelania hupensis lindoensis, snail intermediate hosts Schistosoma japonicum from Central Sulawesi, Indonesia. Biodiversitas 23: 5989-5994. Schistosomiasis is a zoonotic parasitic disease with japonicum. The spread O. lindoensis habitat was found in the three areas Napu, Bada, and Lindu Highlands an infection rate above 1%. primarily cryptic species that are morphologically difficult to identify distinguish other species. Consequently, it can be confused naming One molecular approaches used spp. quickly accurately DNA barcoding using COI mitochondrial gene. However, research on identifying Indonesia still very limited. Therefore, this study aimed six Napu Lindu, Sulawesi gene as marker for barcoding. method Polymerase Chain Reaction (PCR) universal primers, LCO-F HCO-R. data obtained were then analyzed GeneStudio, DNASTAR, BLAST, Identification Engine, Mesquite, MEGAX, BEAST. analysis conducted obtain similarity, genetic distance reconstruct phylogenetic tree. result revealed all samples collected identified one species, namely lindoensis. This important carried out regularly periodically so basis control program related sectors eradicate snails effectively, efficiently, target.